We use cookies on this site. By browsing our site you agree to our use of cookies. Close this message Find out more

Home > Biological Sciences > News, events and resources > Seminars > School Seminar - Dr Ondřej Novák
More in this section Find out more

School Seminar - Dr Ondřej Novák

Location
BLT2
Date(s)
06/12/2017 (15:00-16:00)
Description
Diagram

Hormonal network and methodology

Speaker: Dr Ondřej Novák, Palacký University Olomouc, Czech Republic

Phytohormone metabolite profiling in plant tissues

Our work is focused on the development of high-throughput miniaturized purification methods for minute amounts of plant tissue (1-10 mg). Two novel approaches, a simple one-step purification protocol based on in-tip microSPE (micro Solid-Phase Extraction) and a class-specific miniaturized immunoaffinity chromatography method were utilized. In order to verify the effect of complex sample matrix of the whole method as well as its efficiency and analytical accuracy, the appropriate stable isotope-labelled internal standards have been prepared by the organic synthesis. We have also developed a new method based on ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for targeted profiling of more than 100 analytes, members of the main classes of plant hormones like cytokinins, auxins, brassinosteroids, gibberellins, jasmonates and abscisates and salicylic acid. Use of our procedures can allow the quantification of plant hormones and their derivatives in very limited amounts of plant material. The new and modern analytical approaches make possible a new direction in plant hormone metabolite profiling. We believe that UHPLC-MS/MS technology can be used for fast and sensitive quantitative analysis showing reproducibility in the plant hormone profiling in different plant tissue and cell extracts.

Host: Prof. Gerhard Leuber


   
 
 
 

Comment on this page

Did you find the information you were looking for? Is there a broken link or content that needs updating? Let us know so we can improve the page.

Note: If you need further information or have a question that cannot be satisfied by this page, please call our switchboard on +44 (0)1784 434455.

This window will close when you submit your comment.

Add Your Feedback
Close